产L-丝氨酸菌株SYPS-062的鉴定及碳源对发酵的影响

Identification of L-Serine Producer SYPS-062 and the Effect of Different Carbon Source

摘要:

采用形态学、生理生化实验和16S rDNA序列分析的方法对从自然界中筛选得到的一株能直接利用糖质原料发酵生产L-丝氨酸菌株SYPS-062的分类地位进行了研究, 确定其为谷氨酸棒杆菌(Corynebacterium glutamicum)。同时考察了碳源对菌株SYPS-062发酵产L-丝氨酸的影响, 实验结果表明, 当蔗糖浓度为60 g/L时, 菌株SYPS-062生物量和L-丝氨酸的积累均达到最大值, 分别为8.1 g/L和6.6 g/L。

关键词:L-丝氨酸, 谷氨酸棒杆菌, 鉴定, 糖质原料, 直接发酵

Abstract:

SYPS-062 was an L-serine producing strain stored by our lab, which could directly produce L-serine from sugar. According to morphology, physiological and biochemical identification and 16S rDNA sequence, SYPS-062 was identified as Corynebacterium glutamicum. And the effect of different carbon source for the SYPS-062 and Corynebacterium glutamicum ATCC13032 fermentation were studied. The experiment result showed that the sucrose was 60 g/L, the yield of L-serine and biomass reached the maximal value, 6.6 g/L and 8.1 g/L respectively.

Key words:L-serine, Corynebacterium glutamicum, Identification, Sugar, Direct fermentation