核糖体rDNA序列分析在丛枝菌根真菌研究中的应用

Application of rDNA Sequence Analysis in the Study of Arbuscular Mycorrhizal Fungi

摘要:

丛枝菌根真菌(AMF)是一类古老、专性活体营养的共生菌物, 尚未获得纯培养, 在一定程度上限制了人们对AMF的深入研究。以DNA分析技术为基础的分子生物学技术增加了AMF检测的敏感性与特异性, rDNA序列的同源性和变化性可更真实地反映物种之间的亲缘关系及进化地位, 因而被广泛应用于AMF分类、鉴定、遗传、生态及物种多样性等研究中。本文简要综述了rDNA序列分析技术在AMF系统发育、分子检测及群落结构特征研究中的应用现状。

Abstract:

Arbuscular mycorrhizal fungi(AMF) are ancient, asexual, and obligate symbiotic endophytes which have not been cultured in vitro. So there is some limitation in the study of mycorrhizology. While the molecular technology based on DNA analysis could increase the detection sensitivity and specificity of AMF. rDNA sequence homology and variability can reveal the relationship between species and their evolution. Thus rDNA sequence analysis are widely used in the classification, identification, genetic, ecology and biodiversity of AMF. This article summarizes the rDNA sequence analysis techniques and their application in phylogeny, molecular detection and community structure of AMF in different plant vegetation.