假丝酵母YQ5产S-腺苷甲硫氨酸的发酵培养基优化

Optimization of Fermentation Medium for S-adenosylmethionine Production by Candida sp. YQ5

摘要:

利用单因子实验和正交实验对假丝酵母(Candida sp.)突变菌株YQ5摇瓶发酵产生S-腺苷甲硫氨酸的培养基成分进行了优化。单因子实验结果表明, 发酵最适pH值为6.0, 最佳碳源为 8%蔗糖, 最佳氮源为1.5%胰蛋白胨, 酵母粉最适浓度为2%, MgSO4·7H2O、CaCl2、FeSO4·7H2O、CoCl2、CuSO4·5H2O、H3BO3等作为无机离子对胞内S-腺苷甲硫氨酸的积累均有促进作用。利 用正交实验获得了最终的发酵培养基配方。在最适条件下发酵48 h, S-腺苷甲硫氨酸含量可达1740.0 mg/L。

Abstract:

The optimized cultural medium of fermentation for Candida sp. mutant strain YQ5 to produce S-adenosylmethionine was studied. The results of single factor experiment showed that the most favorable pH value, carbon source, nitrogen source organic nutrient is 6.0, 8% sucrose, 1.5% tryptone and 2% yeast extract, respectively. As to inorganic salts, MgSO4·7H2O, CaCl2, FeSO4·7H2O, CoCl2, CuSO4·5H2O, H3BO3 could improve accumulation of the intercellular SAM. The ingredients of the culture medium are also optimized by the orthogonal experiment. Fermentation for 48 h under the optimal condition resulted in AdoMet production at 1740.0 mg/L.