高效氯氰菊酯降解菌株HG-P-01的培养基筛选及优化

Screen and Optimization of Cultured Medium Constituents for Biodegradation of β-cypermethrin, a Synthetic Pyrethroid Insecticide, by Fusarium sp. Strain HG-P-01 via Central Composite Rotatable Design

摘要:

通过比较查彼氏培养基、马铃薯葡萄糖培养基、理查德培养基、燕麦片培养基和玉米粉液体培养基等5种培养基对高效氯氰菊酯降解菌株Fusarium sp. HG-P-01生长的影响, 确定了查彼氏培养基是菌丝生长最适宜的培养基。采用中心组分旋转设计技术, 优化了查彼氏培养基中Ｃ、Ｎ、Ｐ的组成, 以降解率为衡量指标, 最佳配方包括Ｃ为20.94 g/L、Ｎ为1.82 g/L、P为1.66 g/L, 处理后24 h对50 mg/L高效氯氰菊酯期望降解率96.34%, HPLC法测定实际降解率为93.78%, 对高效氯氰菊酯的降解率模型预测值与实测值一致。

Abstract:

By means of comparing biomasses of biodegradation fungi, Fusarium sp. HG-P-01 for β-cypermethrin, a synthetic pyrethroid insecticide used widely in China, in five different media, the Czapek-Dox medium was selected as the best medium for mycelia growth. Furthermore, an experiment of central composite rotatable design (CCRD) was used to optimize the content of nutrient components. The optimal composition of C, N and P in media for HG-P-01 were 20.94 g/L, 1.82 g/L and 1.66 g/L, respectively, in which an expectant or real rate of β-cypermethrin-degradation got to 96.34% or 93.78% by HPLC for a concentration of 50 mg/L after 24 h treatment. The predicted value in degradation rate model was consistent with that from HPLC method.