高效微生物絮凝剂产生菌TJ-3的絮凝特性分析

Analysis on Flocculation Characteristics of Bioflocculant-producting Bacteria TJ-3

摘要:

从活性污泥中分离筛选出一株高效絮凝剂产生菌TJ-3, 经生理生化试验检测其属于革兰氏阴性菌, 短杆状, 16S rDNA测序鉴定为铜绿假单胞菌。生长曲线表明, TJ-3的生长稳定期较长, 所产微生物絮凝剂(MBF)的稳定性良好。TJ-3产MBF对高岭土悬液具有良好的絮凝效果, 最佳条件下的絮凝率为98.2%。絮凝活性分布实验结果表明, TJ-3所产MBF的活性物质大部分存在于离心后的沉淀物中。处理100 mL高岭土悬液, pH值8.5、1%(质量分数)CaCl2溶液投加量3.5 mL、菌液投加量1.5 mL时, 絮凝效果最佳。

Abstract:

A strain of high-efficiency bioflocculant-producting bacteria, which was named TJ-3, was screened from sewage sludge. The strain was gram-negative and rod-shaped in physiological biochemical test and was identified as Pseudomonas aeruginosa by 16S rDNA Sequencing. According to the growth curve of the TJ-3 strain, the growth stabilization period is long so that Microbial Flocculants (MBF) production is stable. The MBF produced by the TJ-3 strain is able to flocculante kaolin suspension with 98.2%. The MBF activity distribution tests show that most of the active components of the bioflocculant exist in deposition after centrifufation. When pH is 8.5, 1% (quality fraction) CaCl2 Solution dosing quantity is 3.5 mL, bioflocculant dosing quantity is 1.5 mL in 100 mL kaolin suspension, the bioflocculant has an optimal flocculation.