一株高效苯酚降解菌的选育及降酚性能研究

Breeding of the High Phenol-degraded Bacterium JY01 and Study on Phenolic Biodegradation

摘要:

从一绝缘材料厂的污水中分离得到一株可高效降解苯酚的菌株JY01, 该菌株的形态和理化特征与Bacillus基本相同, 其16S rDNA序列与Bacillus simplex (AM9216370)的相似性为99.01%。在接种量为2%的条件下, 该菌在pH为6.0~9.0和温度为18°C~36°C的范围内保持对苯酚良好的降解能力; 30 h内, 当苯酚浓度为1100 mg/L和1300 mg/L时, 其降解率分别为99.16%和74.76%。这将为进一步采用生物法处理含酚废水提供了可靠的控制条件。

关键词:芽孢杆菌, 16S rDNA, 苯酚, 生物降解

Abstract:

A strain JY01 (Bacillus sp. JY01) isolated from wastewater of insulating materials plant had strong ability of phenol degradation. The morphological and physiological characteristics of JY01 were almost the same as Bacillus. The sequence alignment algorithm of the 16S rDNA gene of JY01 shared 99.01% similarity with the typical strain Bacillus simplex (AM921637). Under the conditions of incubation amount of 2%, it could preserve high degradation activities of phenol in the range of the pH 6.0~9.0 and the temperature 18°C~36°C. With the phenol concentration of 1100 mg/L and 1300 mg/L, the degradation rates were 99.16% and 74.76%, respectively. The experiment offered a comparatively reliable parameter for the phenolic wastewater treatment.

Key words:Bacillus, 16S rDNA, Phenol, Biodegradation