一株轻度嗜盐反硝化细菌的分离鉴定和反硝化特性初探

Isolation, Identification and Degradation Characteristics of a Slight Halophilic Denitrifying Bacteria

摘要:

从处理高盐度废水的成熟活性污泥中分离筛选得到1株轻度嗜盐反硝化细菌GYL, 通过对该菌株的形态观察、生理生化实验以及16S rDNA序列分析, 确定该菌株为盐单胞菌(Halomonas sp.)。该菌株能在盐度为10%的培养液中生长, 最适盐度为2%~7%, 最适pH为7.5~8.5, 最佳碳源为蔗糖, 在25°C~30°C的温度范围内脱氮效率达到80%以上。对该菌株的异养硝化能力进行了测定, 其对氨氮的去除率可达98.3%, 说明该菌株可实现同步硝化反硝化, 即该菌可以独立完成生物脱氮的全部过程。

Abstract:

A slight halophilic denitrifying bacteria (designated GYL) was screened from the activated sludge which was used to treat high-salinity wastewater. According to the results of morphological observation, physiological and biochemical test, sequence analysis of the 16S rDNA, strain GYL was identified as Halomonas sp.. This strain could survive at 10% salinity and the optimal salinity range for growth was 2%~7%. The suitable pH value for growth was 7.5~8.5 and sucrose was the most effective carbon source. The nitrogen removal efficiency exceeded 80% when the temperature ranged from 25°C to 30°C. Meanwhile heterotrophic nitrification characteristics of this strain were measured. Results showed that this strain was able to realize SND and ammonia removal rate was 98.3%. It showed that this strain could perform the whole process of bacteria denitrification independently.