三株沙漠放线菌XJSS-21、XJSS-39和XJSS-58的多相分类及特性研究

Polyphasic Taxonomy and Characteristics of Three Desert Actinomycosis XJSS-21、XJSS-39 and XJSS-58

摘要:

从新疆吐鲁番鄯善县典型沙漠地区中采集的样品中分离获得3株放线菌菌株XJSS-21、XJSS-39和XJSS-58, 对其形态特征、生理生化特性、反硝化能力、细胞壁化学组分以及16S rRNA基因序列等进行了多相分类研究, 发现3种沙漠放线菌均能产生纤维素酶, 淀粉酶和蛋白酶, 且菌株XJSS-21具有很强的反硝化能力, XJSS-58属于轻度耐盐菌。初步确定XJSS-21可能属于Streptomyces humidus subsp. antitumoris, XJSS-39可能属于Nocardiopsis dassonvillei, XJSS-58可能属于Streptomyces mutabilis。

Abstract:

The experiment isolated three actinomycete strains XJSS-21, XJSS-39 and XJSS-58 from soil sample collected from typical desert areas in Shanshan County, Turpan City, Xinjiang Province. Based on the polyphasic studies, including its morphology, physiological and biochemical characteristics, denitrifying capability, chemotaxonomy and 16S rRNA gene sequence analysis, we found that three desert actinomycetes are able to produce cellulase, amylase and protease, the strain XJSS-21 has a strong capacity to denitrification, XJSS-58 belongs to the mild salt-tolerant bacteria. XJSS-21, XJSS-39 and XJSS-58 were primarily identified as Streptomyces Humidus subsp. antitumoris, Nocardiopsis dassonvillei and Streptomyces mutabilis.