劳盆地深海热液喷口沉积物中细菌多样性研究

The Diversity of Bacteria in Sediments from Lau Basin Deep-sea Hydrothermal Vents

摘要:

采用PCR-RFLP技术调查了劳盆地深海热液喷口两位点沉积物中的细菌多样性。结果表明, 在劳盆地深海热液喷口沉积物环境中细菌多样性十分丰富, 样品DY1中发现6个细菌类群, DY2中则存在4个细菌类群, 其中Gammaproteobacteria细菌亚群和Epsilonproteobacteria细菌亚群在两文库中均占据最大比例, 为沉积物样品中的优势菌群。另外, 在克隆文库中还发现了一些与数据库中的已知序列同源性较低的类群, 从而说明劳盆地深海热液喷口沉积物中存在特有的微生物种属。

关键词:细菌多样性, 劳盆地深海热液喷口, PCR-RFLP, 系统发育分析

Abstract:

The bacterial diversity of two deep-sea sediments collected at Lau Basin hydrothermal vents were analysed by performing a restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences. Analysis of sequences showed that there were a diversity of bacterial populations in sediments from Lau Basin deep-sea hydrothermal vents, and six bacterial divisions and four bacterial divisions represented in the sample DY1 and DY2 respectively. The phyla Gammaproteobacteria and Epsilonproteobacteria were the most dominant groups in both clone library DY1 and DY2. Additionally, some bacterial sequences had lower similarity to most known sequences in the database, which suggested that there were some novel taxa of bacteria in sediment from Lau Basin deep-sea hydrothermal vents.

Key words:Bacterial diversity, Lau Basin deep-sea hydrothermal vent, PCR-RFLP, Phylogenetic analysis