Dietzia sp. S-JS-1利用废弃油脂生产生物破乳剂的研究

Production of Biodemulsifier by One Strain of Dietzia sp. S-JS-1 in the Use of Waste Frying Oils

摘要:

生物破乳剂是近期开发出来的用于油水分离的新型破乳剂。本研究利用从受石油污染的土壤中筛选得到、并采用16S rRNA鉴定为Dietzia sp.的一株破乳剂产生菌, 在以废弃油脂MWFO、SWFO为碳源培养下, 得到的生物破乳剂的粗重为4 g/L、3.5 g/L; 对于W/O、O/W模型乳状液的破乳效果均可超过以液体石蜡产生的破乳剂, 且以SWFO废弃油脂培养得到的生物破乳剂可以同时应用于两种模型乳状液的使用。对于碳源利用方面Dietzia sp.在利用两种废弃油脂脂肪酸的过程中, 都是优先利用C16和C18的脂肪酸, 但对于两种废弃油脂的利用率上存在一定差异。采用TLC和FTIR分析发现, 3种碳源培养得到的生物破乳剂均为脂肽类生物破乳剂, 其破乳剂的化学结构还有待进一步研究。

关键词:Dietzia sp., 废弃油脂, 生物破乳剂, 脂肽

Abstract:

As a new demulsifier, bio-demulsifier is explored recently to break the oil-water emulsion. In this study, one demulsifiying strain isolated from oil contanimated soil was identified as Dietzia sp. by 16S rRNA. When it was cultured in MMSW substrate, adding MWFO and SWFO as the carbon source, it could produce crude biodemulsifier of 4 g/L and 3.5 g/L respectively. Compared with biodemulsifier produced by paraffine as the carbon source, both of them could achieve higher demulsification ratio in breaking W/O and O/W model emulsion. Additionally, biodemulsifer produced by SWFO as the carbon source had the ability to demulsify two model emulsion effiectively. By analyzing the carbon utilization, it could be concluded that C16 and C18 fatty acid of the two waste frying oils were preferable for Dietzia sp.. The biodemulsifers produced from three different carbon sources all of which were identified as lipopeptide by TLC and FTIR. Further research should be taken to investigate its chemical structure.

Key words:Dietzia sp., Waste frying oil, Bio-demulsifier, Lipopeptide