Based on improving experimental operating skills of students and considering the features of microbiology experimental technology and methods, we developed a new examination ways of microbiology experiment — “multimedia papers of microbiology experiments” to use among 2005 and 2006 students of sophomore(entering campus in 2005 and 2006) of biotechnology and bio-science for microbiology experiment examination. Analysis of the results of experiment examination and theoretical examination for the 2005 and 2006 students showed that the disharmony and miscorrelation between experiment examination results and theoretical examination results were overcome, and students got more interested in experimental classes, while their experimental operation is more conscious and accurate.