一株杀黄孢链霉菌IMB-14的分离与鉴定

The Isolation and Identification of Strain Streptomyces xanthocidicus IMB-14

摘要:

采用薄膜过滤法从武当山采集土壤中分离到一株具有广谱抗菌活性的链霉菌, 命名为IMB-14。通过对其抗菌活性、形态特征、培养特征、生理生化特性和16S rDNA 序列的多相分类研究, 结合代谢产物的性质鉴定该菌株为杀黄孢链霉菌(Streptomyces xanthocidicus)。该杀黄孢链霉菌的分离鉴定为新型抗菌及其抗癌活性物质的筛选奠定了基础。

关键词:薄膜过滤法, 多相分类, 杀黄孢链霉菌, 抗生素FR-900148

Abstract:

The strain Streptomyces sp., nominated IMB-14, was isolated from the soil sample of WuDang Mountain by the method of cellulose ester membrane filter. The studies on antibiotic activities, morphological characteristics, cultural characteristics, physiological characteristics, 16S rDNA sequence analysis and the metabolite of strain IMB-14 showed that the strain IMB-14 was accordance with Streptomyces xanthocidicus. The study on the isolation and identification of strain Streptomyces xanthocidicus establishes a foundation on screening of novel antibacterial and antitumor agents.

Key words:Cellulose ester membrane filter method, Polyphasic taxonomy, Streptomyces xanthocidicus, Antibiotic FR-900148