(1. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China) (2. Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of Agriculture, Institute of Agro-food Science and Technology, Chinese Academy 在期刊界中查找 在百度中查找 在本站中查找
(1. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China) (2. Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of Agriculture, Institute of Agro-food Science and Technology, Chinese Academy 在期刊界中查找 在百度中查找 在本站中查找
In this study, a mutant designated NC1150 which dose not produce streptolysin was selected from wild-type Streptococcus equisimilis by ultraviolet ray combined with 60Co-γ ray mutation. Through further 60Co-γ ray mutation a high-producing hyaluronic acid mutant designated NC168 was obtained. This selected mutant NC168 could produce hyluronic acid 1.01 times as much as the original strain NC1150 and relative molecular weight of hyaluronic acid touched 0.55×106 D. Furthermore, its hyaluronic acid output and relative molecular weight of hyaluronic acid maintained genetic stability after 10 generations and there was no reverse mutation on hemolysis.
