螺旋藻对腹泻模型小鼠肠道菌群的影响

The Effects of Spirulina on Intestinal Microflora of Diarrhea Model Mice

摘要:

为评价螺旋藻对肠道菌群的影响, 采用注射氨苄青霉素的方法, 制造小鼠腹泻模型, 造模成功后灌服不同剂量螺旋藻进行治疗, 取不同治疗时间的粪便样品, 检测双歧杆菌、乳杆菌、大肠杆菌、肠球菌、拟杆菌、产气荚膜梭菌菌群的数量,并在螺旋藻灌胃8 d后, 测定小鼠不同肠段各指标菌群的数量。结果表明, 中高剂量组螺旋藻对腹泻模型小鼠肠道菌群有明显调整作用, 有效缩短了肠道菌群由失调到平衡的时间；粪便样品同各肠段内容物所反映的菌群的变化趋势相同, 即治疗恢复组的双歧杆菌、乳杆菌数量总体上高于生理盐水组, 肠杆菌、肠球菌、拟杆菌、产气荚膜梭菌数量均低于生理盐水组, 但取样位置不同, 各指标菌群的数量明显存在差异。

Abstract:

The effects of Spirulina on intestinal microflora of diarrhea model mice were observed. Diarrhea model mice were geted by gastric perfusion ampicillin, then these mice were given different doses spirulina by gavage. Selective culture media were used to culture bifidobacteria, bacterium lacticum, enteric bacilli, enterococci, bacteroid, clostridium perfringens in feces at different times. In order to find the difference of collecting samples from feces and intestine, the quantity of detection flora in different segment of intestine were compared too. The result showed that in middle dose group and high dose group spirulina can regulate and improve intestinal flora of diarrhea model mice, and effectively shorten time from disturbance to balance. Fecal sample and intestinal contents sample both manifest a same trend, that is the quantity of bifidobacteria, bacterium lacticum in treatment recovery group is significantly higher than saline group, and enteric bacilli, enterococci, bacteroid, clostridium perfringens is significantly lower than saline group, but each index flora in intestinal contents sample has a significant difference in quantity.