拮抗辣椒疫病菌的红树内生细菌筛选及RS261菌株鉴定

Screen of Mangrove Endophytic Bacteria Antagonists Against Phytophthora capsici and Identification of Strain RS261

摘要:

红树内生细菌分离及拮抗辣椒疫霉(Phytophthora capsici)筛选结果表明：各红树体内均有大量的内生细菌, 不同红树种类及部位内生细菌的数量均不同, 被测定的红树内生细菌中约有27.97%的可培养菌株对辣椒疫霉具有拮抗作用; 其中18株拮抗作用较强的细菌在辣椒果上对辣椒疫病菌均有一定的抑制效果, 以来自红海榄叶片内的RS261菌株效果最好; 经形态、生理生化特征和分子生物学等测定分析, 将RS261菌株初步鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。

Abstract:

The isolation of the endophytic bacteria from mangroves and screen of the antagonistic bacteria against the Phytophthora capsici were studied in this paper. The results showed that there were a large number of the endophytic bacteria in the tissues of mangroves. Of the endophytic bacteria, about 27.97% strains expressed the inhibitive activities to P. capsic. Using the eighteen antagonistic bacteria to control pepper fruit Phytophthora blight showed that all the tested strains had some control efficacy to the disease. In them, strain RS261 isolated from the leaf of Rhizophora stylosa was found to have the best control efficacy. Based on the morphological, physiological and biochemical characteristics and on 16SrDNA sequences analysis, strain RS261 was identified as Bacillus amyloliquefaciens.