The degradation of nitrobenzene(NB) using a combination of Fe0 and anaerobic microorganism was studied. Nitrobenzene could be degraded effectively and the synergistic effect between Fe0 and anaerobic microorganism was apparent, and the nitrobenzene removal efficiency increased with the increasing of Fe0; the optimum pH was 5.0~6.0; as co-metabolizing substrate, glucose could promote the degradation of nitrobenzene; In case of high concentration of Fe2+ and Fe3+, the anaerobic biodegradation activity of nitrobenzene were inhibited in a certain degree; 0.5 mg/L Fe2+ and Fe3+ were the optimum to accelerate biodegradation rate of nitrobenzene; the degradation kinetics of nitrobenzene were followed by first-order reaction, reaction rate constant reduced along with the concentration of nitrobenzene increased.
