石楠拟盘多毛孢毒素产生条件初探

Preliminary Research on Conditions of Toxins Produced by Pestalotiopsis photiniae

摘要:

石楠拟盘多毛孢是引起草莓根腐病的优势病原菌之一, 本实验在明确该病菌毒素是主要致病物质的基础上, 利用叶圆片法对该菌的产毒条件进行了初步探讨, 结果表明, 除光照影响不明显外, pH值、温度、振荡及培养时间等对此菌产生毒素均有显著影响, 其最适产毒条件为：培养基初始pH值为自然pH值(约为6.2)、温度为25°C、黑暗、静置培养5 d~7 d。此外, 实验还发现该菌产生的粗毒素对玉米、黑麦和绿豆种子萌发、根或芽的延伸生长都有明显的抑制作用。

Abstract:

Pestalotiopsis photiniae is one of the predominant pathogens of strawberry root rot disease. Based on preliminary research, it was proved that crude toxins were main pathogenic substances of the pathogen. For further investigation and utilization of toxins produced by this fungus, conditions of producing toxins were analyzed with the leaf disk method in this experiment. The result showed that pH values, cultural time, vibration, and tested temperatures obviously affected the production of toxins, except for light treatment. The most suitable culture conditions for the toxin production were pH 6.2, 25°C, darkness and stillness, for 5 d~7 d. Besides, it was discovered that crude toxins could significantly inhibit seed germination and elongation growth of roots or shoots for maize, rye and mung bean.