Cryptococcus curvatus O3酵母菌培养及产油脂特性

The Cultivation and Lipids Accumulation of Cryptococcus curvatus O3 Yeast

摘要:

生物柴油的发展, 导致全球油脂供求紧张。微生物油脂的甘三酯组成与植物油类似, 发展微生物油脂可部分缓解植物油脂供应压力。本文研究了Cryptococcus curvatus O3酵母利用葡萄糖为碳源生长和积累油脂的特性。Cryptococcus curvatus O3酵母在培养过程中能适应间歇式碳源流加方式达到高密度培养的目的, 但在相同培养条件下, 不同氮源能影响其代谢过程中糖到油脂转化的脂肪系数。Cryptococcus curvatus O3酵母利用葡萄糖作为碳源在30°C下摇瓶发酵, 菌体生物量为51.8 g/L, 油脂含量达65.1%。脂肪酸组成分析结果表明, 菌油富含饱和及低度不饱和长链脂肪酸, 其中饱和脂肪酸之和占总脂肪酸组成的64%左右, 其脂肪酸组成类似于可可脂, 这些结果对于利用产油微生物转化生物质获取如类可可脂等具有高附加值油脂的研究具有重要意义。

Abstract:

Recent demand for biodiesel worldwide has turned vegetable fats into an ever-growing and substantial consumption resource. In this regard, microbial lipids which triacylglycerol(TAG) is similar to the vegetable fats, may be a prospective alternative feedstock for biodiesel industry. Described herein are preliminary screening studies on Cryptococcus curvatus O3 strain for assimilation glucose to produce microbial lipids. Cryptococcus curvatus O3 When cultivated by shaking flask at 30°C with glucose as sole carbon source, cellular biomass and lipid content were 51.8 g/L and 65.1%. Therefor Cryptococcus curvatus O3 strain can be defined as an oleaginous yeast. Gas chromatography analysis of microbial lipids revealed that major constituent fatty acids were palmic acid, oleic acid and linoleic acid. The percentage of SFA (saturated fatty acid)in the lipid of Cryptococcus curvatus O3 is 64%. Such compositional feature was quite similar to cocoa butter, one of the high quality vegetable fats. Therefore, the oleaginous yeast could be considered as an ideal host for fermentation of biomass for lipids.