水体中病毒浓缩方法及其条件优化

Methods of Viruses Concentration and Conditions Optimization in Water

摘要:

本研究探讨了在自来水和污水两种不同的水体环境中, 三氯化铝沉淀法和正电荷滤膜法浓缩回收病毒的效率, 并比较应用不同的浓缩条件、洗脱物质时的病毒回收率, 从而建立有效的环境样本中病毒的浓缩方法。结果表明, 三氯化铝沉淀法在两种水体中的回收率均较高, 最高回收率分别达到96.0%和92.0%, 但正电荷滤膜法在两种水体中的回收率差别较大。在自来水中, 正电荷滤膜法的最高回收率为93.9%, 在污水中的最高回收率仅为69.9%, 这说明正电荷滤膜法适用于病毒含量较高且悬浮物等杂质较少的样本。在应用于自来水样时, 两种方法均可起到有效的病毒浓缩作用, 但在悬浮物等杂质较多的污水中, 三氯化铝法具有较好的回收效率。

Abstract:

The efficacy of the method of AlCl3 sedimentation and positive-charged membrane filtration for concentrating viruses was compared in this study. In addition, the efficacy of the two methods was also compared in different waters, conditions and elution. The recovery rate of the sedimentation method of AlCl3 and the positive-charged membrane was 96.0% and 92.0% respectively when tap water served as samples. The recovery rate of the sedimentation method of AlCl3 and the positive-charged membrane was 93.9% and 69.9% respectively when sewage served as samples. Compared with the two concentration methods, there was no obvious difference in tap water but significant difference in sewage. The results showed that the positive-charged membrane was adapted to the water samples with little impurity. According to the experiment results, the two methods could be used to effectively concentrate viruses in tap water. However, the sedimentation method of AlCl3 was recommended to the primary method in other water samples.