不对称还原苯乙酮的酵母菌株筛选与分子鉴定

Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone

摘要:

利用苯乙酮作为模式底物, 对145株菌株进行初筛和复筛, 获得一株具有高效立体选择性的酵母菌株YS6-2, 能够不对称还原苯乙酮生成(S)-1-苯基乙醇。在苯乙酮浓度为70 mmol/L时, 底物的初始转化率达26.8%, 产物(S)-1-苯基乙醇的对映体过量值为98.8%。基于形态学、生理生化特征、18S rDNA和26S rDNA D1/D2区域的分析表明, YS6-2为胶红酵母(Rhodotorula mucilaginosa)。

Abstract:

Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmetric reduction of acetophenone to (S)-1-phenethylalcohol was chosen as the model reaction. With the concentration of acetophenone at 70 mmol/L, YS6-2 yielded (S)-alcohol at 26.8% conversion and enantiomeric excess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain sequence, along with its general morphology, physiological and biochemical characteristics, YS6-2 was identified as Rhodotorula mucilaginosa.