氯嘧磺隆降解菌株LW-3的分离及生物学特性研究

Isolation of Strain LW-3 Capable of Degrading Chlorimuron-ethyl and Its Biological Characteristics

摘要:

从长期受氯嘧磺隆污染的土壤中分离到一株氯嘧磺隆高效降解菌株, 命名为LW-3, 菌株LW-3可以氯嘧磺隆为唯一氮源生长, 接种量为2%时, 50 mg/L氯嘧磺隆经过7 d, 降解率达70%~80%。生理生化实验和16S rDNA序列同源性分析, 将菌株LW-3归属于假单胞菌属(Pseudomonas sp.); 菌株的适宜降解条件为; 温度30°C~35°C, pH 6.5~7.2, pH对菌株LW-3降解氯嘧磺隆有明显地影响。

关键词:氯嘧磺隆, 微生物降解, 假单胞菌属, 生物学特性

Abstract:

A bacterium(designated as LW-3), capable of degrading Chlorimuron-ethyl, was isolated from the long-term contaminated soil by Chlorimuron-ethyl. Based on physiological and biochemical analyses and sequences comparison of 16S rDNA, strain LW-3 was identified as Pseudomonas sp. LW-3 could use Chlorimuron-ethyl as sole nitrogen source for growth. The optimum pH and temperature for degradation of Chlorimuron-ethyl were pH 6.5~7.2 and 30°C~35°C, at same temperature the pH change to the Chlorimuron -ethyl degrading influence is large. When the pH and temperature were pH 6.5 and 32°C, 50 mg/L Chlorimuron-ethyl could be degraded to 70%~80% level within 7 days.

Key words:Chlorimuron-ethyl, Micro-biodegradation, Pseudomonas sp., Biological characteristics