2,4-二氯苯氧乙酸(2,4-D)对雨生红球藻中虾青素积累的影响

The Impact of Extraneous Salicylic Acid (SA) to Astaxanthin Accumulation of Haematoccus pluvialis

摘要:

本文初步研究了一定浓度范围内的2,4-D对雨生红球藻积累虾青素的影响。在对数生长期的藻液中分别加入一系列不同浓度的2,4-D溶液, 然后进行胁迫培养(25°C、24 h、5000 Lx连续光照+营养盐饥饿), 诱导细胞内虾青素的合成积累。在诱导过程中, 显微观察不同浓度2,4-D处理后细胞形态和虾青素积累的动态变化, 并定期取样测定虾青素含量。结果表明, 20.0 mg/L的2,4-D能够明显促进雨生红球藻中积累虾青素。它不仅可以加快虾青素积累进程(比对照提前15 d), 而且比对照能提高13.4%的虾青素产量。

Abstract:

Effect of extraneous 2,4-D on astaxanthin accumulation in H. pluvialis was studied in this paper. After different concentration grades of 2,4-D were added into the vegetative green cells respectively, then H. pluvialis were cultivated under unfavorable growth conditions (24 h light cycle, 5000 Lx, 25°C, nutrition deficiency) to promote astaxanthin accumulation. In this stage, morphological changes of cells treated by different grades of 2,4-D concentration were observed with optical microscopy. Moreover, astaxanthin content was determined with absorption spectral (OD490). Results showed that astaxanthin accumulation might be promoted at 20 mg/L 2,4-D treatment which could quicken astaxanthin accumulation 15 days compared with blank controls. Moreover, the increasing production extents of astaxanthin reached 13.4% than blank controls.