腾冲热海一株嗜酸热硫化叶菌的分离与鉴定

Identification of a Thermoacidophilic Sulfolobus sp. Isolated from a Hot Spring in Tengchong Rehai

摘要:

从云南腾冲热海酸性温泉中分离纯化出一株极端嗜酸热菌株K4-1, 并对其进行形态观察、生长特征、碳源和能源利用及16S rRNA基因分析。该菌株细胞形态为不规则球形, 有单生鞭毛, 严格好氧, 兼性自养, 能利用元素硫作为能源, 也能利用酵母膏、精氨酸或核糖作为碳源和能源。其最适生长温度为75°C, 最适pH为3.5。通过16S rRNA基因序列相似性对比对该菌株进行鉴定, 结果表明该菌株与硫化叶菌属标准菌株的相似性介于86.6%~94.3%之间, 与分离自腾冲热海的腾冲硫化叶菌Sulfolobus tengchongensis RT8-4菌株序列相似性最高, 达到98.9％, 可将菌株K4-1鉴定为硫化叶菌属菌株。菌株K4-1的16S rRNA基因序列号为EU729124。

Abstract:

An extremely thermoacidophilic isolate K4-1 was obtained from an acidic hot spring in Tengchong Rehai, Yunnan province. Morphology, growth characteristics, utilization of carbon compounds, energy sources and 16S rRNA gene sequence of K4-1 were studied. Cells of K4-1 are irregular cocci with monotrichous flagella. The strain grew aerobically in either a lithotrophic or a heterotrophic mode. Growth on elemental sulfur occurred through oxidation of sulfur. It grew optimally at 75°C and pH 3.5. On the basis of 16S rRNA gene sequence similarity, strain K4-1 was shown to belong to genus Sulfolobus, being related to the type strains of genus Sulfolobus (86.6%~94.3% similarity), and being most closely related to strain Sulfolobus tengchongensis RT8-4 (98.9% similarity). The GenBank accession number of strain K4-1 16S rRNA gene sequence is EU729124.