Malolactic fermentation (MLF) is performed principally by Oenococcus oeni in order to improve the stability and quality for winemaking. MLF usually occurs either spontaneously or after inoculation with selected bacteria. The functions of Saccharomyces cerevisiae, phenolic compounds and winetechnics to Oenococcus oeni were summarized in this review. The effects of wine physic-chemically harsh conditions on the Metabolism of Oenococcus oeni were discussed. We look forward to the information being the references for MLF controlling.