一株家养野猪源猪圆环病毒2型的分离与鉴定

Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar

摘要:

应用PCR方法从临诊疑似断奶仔猪多系统衰竭综合征(PMWS)家养野猪病例的淋巴结和脾脏中扩增出预期长度的猪圆环病毒2型(PCV2)的DNA片段, 在Dulac细胞中进行分离和培养, 扩增全基因组序列后进行同源性分析。结果显示, 扩增产物与家猪源参考毒株的序列同源性均在98%以上, 该病毒与家猪源病毒差异不大。

Abstract:

The nucleic acid fragment of porcine circovirus type 2(PCV2)was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome (PMWS). The sample were then inoculated into the Dulac cells and passaged for 8 generation. The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence. The sequence of genome was compared with other 9 strains originated from piglets in the same area. The result showed that these strains were in high homology.