烟草炭疽病拮抗生防菌的筛选

Screening of Antagonistic Strains to Tobacco Anthracnose

摘要:

本文应用荧光假单胞杆菌的16S-23S ribosomal RNA序列以及部分抗菌素合成基因序列的PCR鉴定引物, 对从烟草根际分离到的400份细菌进行了鉴定, 结果获得了21份含Prn(硝吡咯菌素)合成酶基因的荧光假单胞杆菌和1份含有DAPG(2, 4-二乙酰基藤黄酚)合成酶基因以及PCA (Phenazine-1-carboxylic acid, 吩嗪-1-羧酸)的荧光假单胞杆菌。室内实验结果表明, 含DAPG和PCA的荧光假单胞杆菌对烟草炭疽病具有明显拮抗作用。

Abstract:

Special PCR primers were designed from the sequence of 16S-23S ribosomal RNA of Pseudomonas fluorescens, then these primers were used to screening 400 bacteria strains which selected from tobacco planting soil. In the experiment, 21 Pseudomonas fluorescens strains which contain Pyrrolnitrin had been identified, and 1 Pseudomonas fluorescens strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid had been identified, too. The results showed that the strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid inhibit to tobacco anthracnose.