Field studies were conducted for a season to determine potential for alterations in the rhizosphere fungi quantity dynamics and dominant populations of 4 wild sugarcane clones(S. spontaneum L.) with different UV-B sensitivity under an enhanced ultraviolet-B (UV-B, 280 nm~310 nm) radiation. The quantity of rhizosphere fungi was most in tillering stage, second in seedling stage and lest in elongating stage and maturing stage, the sequence wasn’t alternated by UV-B radiation. The rhizosphere fungi quantity of tolerant clone was obviously increased and greater than the sensitive clone under the enhanced UV-B radiation, however, the number of dominant populations decreased and Penicillium was the dominant population during the periods of 4 wild sugarcane clones.