透明颤菌血红蛋白在产聚γ-谷氨酸地衣芽胞杆菌WX-02中的表达

Expression of Vitreoscilla Hemoglobin in Poly γ-glutamic Acid-producing Bacillus licheniformis WX-02

摘要:

聚γ-谷氨酸(γ-PGA)是一种应用前景良好的生物高分子材料。通过构建含有α-淀粉酶(amyE)基因两端交换臂的整合载体pDG1730-vgb, 将透明颤菌血红蛋白基因(vgb)整合到聚γ-谷氨酸生产菌株地衣芽胞杆菌(Bacillus licheniformis)WX-02染色体中, 获得重组子M2。一氧化碳差光光谱结果验证M2中表达了有活性的血红蛋白, 3 L发酵罐分批发酵结果显示M2的生物量比出发菌株WX-02提高了25.5%, γ-PGA产量提高了20%。

Abstract:

Poly γ-glutamate is a biopolymer material that has a good application prospect. The Vitreoscilla hemoglobin(VHb) gene was integrated into the chromosome of Bacillus licheniformis WX-02 by integrative vector pDG1730-vgb. The expression of VHb was confirmed by CO-difference spectra analysis. It was shown by the results of batch cultures in a 3 L bioreactor that biomass and γ-PGA obtained in the recombinant M2 were 25.5 % and 20% higher than those of the control respectively.