微生物来源的L-阿拉伯糖异构酶的研究进展及应用前景

Research Progress on the Microbial L-Arabinose Isomerase

摘要:

L-阿拉伯糖异构酶(L-AI)能分别催化L-阿拉伯糖和D-半乳糖异构为L-核酮糖和D-塔格糖, 它是目前生物法生产新型功能性因子D-塔格糖最为有效的酶。近年来, L-AI的结构已被揭晓, 其基因已获得克隆、测序和过量表达, 经过蛋白质工程改造的L-AI将是未来工业化生产D-塔格糖的主要用酶。本文综述了近年来国外对L-AI的结构与功能、催化机理、酶学性质及应用于D-塔格糖生产方面的研究状况, 并展望了其发展前景。

关键词:L-阿拉伯糖异构酶, D-塔格糖, 结构与功能, 酶学性质, 基因工程

Abstract:

L-arabinose isomerase (L-AI) can isomerize L-arabinose and D-galactose into L-ribulose and D-tagatose, respectively, which is currently the most effective biological catalyst for D-tagatose production. The crystal structure of L-AI has been solved recently and its gene has been cloned, sequenced and overexpressed. L-AI improved by protein engineering will be the dominant enzyme for industrial production of D-tagatose. This paper reviewed researches on protein structure and function, properties and application in D-tagatose production of L-AI, and the long-term potential development of L-AI was prospected.

Key words:L-arabinose isomerase, D-tagatose, Structure and function, Enzyme properties, Genetic engineering