H9N2亚型AIV排毒及形成气溶胶的研究

Studies on Shedding Virus and the Formation of Aerosol of H9N2 Subtype AIV

摘要:

为研究H9N2亚型AIV排毒及形成气溶胶规律, 将SPF鸡饲养于正负压隔离器中, 采用AGI-30收集器(All Glass Impinger AGI- 30)和气管泄殖腔棉拭子在攻毒后不同时间收集空气、气管和泄殖腔样品, 并利用HI、Dot-ELISA和RT-PCR检测样品。结果发现攻毒后第4天开始形成气溶胶, 并持续到第43天, 实验证明H9N2亚型AIV不仅可以在呼吸道和泄殖腔复制, 而且可以形成气溶胶。气管泄殖腔棉拭子在接种后第3天开始排毒, 至第7天攻毒鸡全部分离到病毒。排毒时间可持续到第45天。但泄殖腔的排毒量明显低于气管的排毒量, 这也说明H9N2型禽流感主要通过呼吸道排毒。

Abstract:

In order to study the regularity of shedding virus from infected SPF chickens and the formation of aerosol of H9N2 subtype AIV, SPF chickens were bred in a positive and negative pressure isolator. Aerosol samples were collected by AGI-30 (All Glass Impinger-30) extractor, and simultaneously trachea and cloaca samples were collected by tracheal swabs and cloacal swabs in different periods after challenged with viruses. The above-mentioned samples were detected by HI, Dot-ELISA and RT-PCR methods. The results indicated that aerosols were isolated from the 4 days to the 43 days after inoculation. It was proved that H9N2 subtype AIV could copy themselves in respiratory passage and cloaca, and then could formation of aerosols. AIV H9N2 subtype could be isolated from cloacal and tracheal swabs 3 days after inoculation and lasted for 45 days, viruses were detected from all infected SPF chickens on 7 days.