根际益生菌链霉菌S506固体发酵条件优化

Optimum of Solid Fermentation Parameters for Streptomyces sp. S506

摘要:

链霉菌S506是一株具有广谱防病、促生和降解根系毒素功能的根际益生菌。采用液固两相发酵工艺, 以活菌总数和分生孢子产生量为检测指标, 研究了S506活菌制剂固体发酵物料的配方和工艺参数。获得固体发酵物料的最佳参数为：麸皮100 g、M-1 15 g、磷酸氢二钾0.3 g、氯化钠0.25 g、硝酸钾0.1 g、碳酸钙0.8 g, 自然pH值, 液体菌种种龄84 h, 接种量10%, 发酵温度30°C, 料水比10:6, 发酵时间60 h。在最佳发酵条件下, S506固体菌剂的活菌总量达到8.27×109 CFU/g, 分生孢子数达到6.23×109 CFU/g。

Abstract:

Streptomyces sp. S506 is one of PGPR strains isolated from tomato rhizosphere. The optimum solid fermentation parameters were studied. According to the amount of alive cells and the rate of conidium production, the optimum parameters were obtained as follows: bran 100 g, mineral matter M-1 15 g, K2HPO4 0.3 g, NaCl 0.25 g, KNO3 0.1 g, CaCO3 0.8 g, natural pH, liquid seed age 84 h, inoculum volume 10%, fermentation temperature 30°C, the ratio of material to water is 10:6, fermentation time 60 h. Under the optimized conditions, the alive cells amount of S506 will reach to 8.27×109 CFU/g, and the number of conidium is 6.23×109 CFU/g.