中国红豆杉内生细菌的分离鉴定及活性研究

Isolation, Identification and Bioactivity Assays of Endophytic Bacteria Associated with Taxus chinensis

摘要:

从中国红豆杉的茎中分离得到两株内生细菌G18、F19, 通过生物学特性和16S rDNA序列分析, 初步鉴定这两株菌分别为假单胞菌属(Psudomonas)和寡养单胞菌属(Stenotrophomonas)细菌。活性研究表明, G18、F19发酵液均对3种病原细菌有抑制作用, 分别对棉花黄萎病菌(Verticillium dahliae)和柑橘炭疽病菌(Colletotrichum gloeosporioides)有较强的抑制作用。G18和F19分别能降解水杨酸和敌敌畏。

Abstract:

Two endophytic-bacteria isolates of G18 and F19 were isolated from the stem of Taxus chinensis. The G18 and F19 were respectively classified into Psudomonas sp. and Stenotrophomonas sp. based on biological characteristics and 16S rDNA sequence analysis. The bioactivity analysis showed that the fermented broths of the G18 and F19 exhibited antagonistic activities against three pathogenic bacteria, and had good antagonistic effectiveness to Verticillium dahliae and Colletotrichum gloeosporioides, respectively. The G18 can degrade salicylic acid, and the F19 can do dichlorvos.