假单胞菌荧光与非荧光铁载体对铁离子的应答差异

Different Response of Dluorescent and Non-fluorescent Siderophores from Pseudomonas to Ferric Iron

摘要:

假单胞菌既能产荧光铁载体也能产非荧光铁载体。通过对假单胞菌在不同铁离子浓度下, 在通用CAS(Chrome azroul S)检测平板、改进的蔗糖-天冬氨酸(SA)平板(MSA)上以及通用液体CAS培养基和MSA培养基内的铁载体产生情况的比较, 发现在通用CAS的液体培养基上产生的主要为非荧光铁载体(pyochelin), 而在改进的MSA培养基上产生的主要为荧光铁载体(pyoverdine); 在铁离子的应答方面, pyoverdine较pyochelin灵敏, 较低的铁离子浓度即可抑制荧光铁载体的产生, 但是不能抑制非荧光铁载体。

关键词:铁载体, Pyochelin, Pyoverdine, 铁离子, 假单胞菌

Abstract:

We have studied the influence of ferric iron on the production of siderophore (microbial iron transport compound) from Pseudomonas sp. on universal CAS (Chrome azroul S) assay agar and modified sugar-aspartic acid (MSA) agar or liquid medium respectively. The results showed that the non-fluorescent siderophores (pyochelin) were produced on universal CAS assay agar and liquid medium and fluorescent siderophores (pyoverdine) on MSA agar and liquid medium; and the pyoverdine was more sensitive to the response of ferric iron whatever on agar plate or in liquid medium.

Key words:Siderophore, Pyochelin, Pyoverdine, Iron(Ⅲ), Pseudomonas