水稻植物内生链霉菌中线型和环型质粒的检测

Detection of Linear and Circular Plasmids Among Endophytic Streptomyces Strains from Rice Plant

摘要:

以广东番禺和五山地区水稻植株中分离到的内生链霉菌为对象, 调查可能存在的内源质粒。利用脉冲电泳技术从8个菌株中检测到大小在60 kb~410 kb的线型质粒, 其中4个菌株的线型质粒可能有保守的端粒复制基因。该结果与土壤链霉菌中检测到线型质粒和具有保守端粒复制基因的比例相似, 表明水稻植物组织内部的独特环境不会造成链霉菌线型质粒的多样性分布产生大的变化。此外, 从13个菌株中检测到6 kb~60 kb的环型质粒。

Abstract:

Linear and circular plasmids were investigated among the 44 strains of the rice plant endophytic Streptomyces. By pulsed-field gel electrophoresis, 60 kb-410 kb linear plasmids were detected from the 8 strains and four of which might contain the conserved telomere replication gene tap, resembling that of soil Streptomyces strains, indicating that the diversity of Streptomyces linear plasmids was not affected by the unique environment of the rice plant. By alkaline method, 6 kb~60 kb circular plasmids were detected on gel from the 13 strains.