好氧反硝化菌的选育及其初步应用

Selection of an Aerobic Denitrifying Bacterium and Its Preliminary Application in Aquarium Water

摘要:

采用间歇曝气、极限稀释和酸碱指示剂培养基相结合的办法, 从池塘底泥中成功分离到好氧反硝化菌株H2, 经生理生化和16S rDNA序列分析, 初步判断为芽孢杆菌属(Bacillus sp.)。在室内模拟养殖水体中, 菌株H2在15 d中对亚硝酸盐和硝酸盐的最高降解速率分别达到0.885 mg/L×d和0.46 mg/L×d, 试验结束时, 总氮去除率达到45.2%。结果表明, 菌株H2具有应用于养殖水体生物脱氮领域的巨大潜力。

Abstract:

An aerobic denitrifying bacterium H2 was selected from the pond sludge by using a new screening method: Samples were enriched by intermittent aeration firstly, secondly limiting diluted, then screened with BTB screening culture medium. Strain H2 was identified as Bacillus sp. according to its physiological and biochemical characters, as well as 16S rDNA sequence homology comparison. The highest degradation velocity of NO3- and NO2- were 0.46 mg/L×d and 0.885 mg/L×d, and the degradation rate of total nitrogen was 45.2% in aquarium water during the 15-day experiment. The study showed that strain H2 had great potential in the biodenitrification of aquaculture.