Abstract:Microbial technology is a potential new technology in new energy development process and it has important theoretical and practical significance on the sustainable development of energy sources. The principles, present situations, advantages and disadvantages of new energy sources, such as biodiesel, fuel ethanol, biological methane production, biological hydrogen production and microbial fuel cell were reviewed. The applications of microbial sources in energy field were summarized. Finally, some research emphases such as discover new microbe sources, construct gene engineering microbes, definitize effect mechanism and exploit new technologies were given.