海南粗榧内生真菌的分离与初步鉴定

Isolation and Primary Identification of Endophytic Fungi from Cephalotaxus hainanensis L.

摘要:

从海南粗榧(Cephalotaxus hainanensis L.)健康的树皮、枝、叶中分离得到72株内生真菌。依据其菌落形态和显微特征, 将其中68株归为半知菌类, 即产孢的33株鉴定为5个属, 未产孢的35株中除1株鉴定为丝核菌属外, 其余34株暂归为无孢菌群。还有4株属于担子菌纲。结果表明, 海南粗榧内生真菌对宿主具有一定的组织专一性, 树皮的真菌在数量、种群及其组成方面与枝、叶的相比存在较大差异。

Abstract:

Seventy-two strains of endophytic fungi were isolated from the healthy bark, branches and leaves of Cephalotaxus hainanensis L.. Sixty-eight of them were morphologically classified into Fungi Imperfecti, thirty-three sporulated were identified to five genera. For those did not sporulate, one was identified to Rhizoctonia sp., the rest were tentatively classified into Mycelia Sterilia. Four were identified to Basidiomycetes. The result indicated the endophytic fungi of C. hainanensis show a degree of tissue specificity. There were significant differences about the quantity, genera and composition between the fungi isolated from bark and those from branches and leaves.