新疆胀果甘草内生细菌枯草芽胞杆菌产甘草酸类代谢产物的初步研究

Preliminary Studies on Its Glycyrrhizinic Acid Metabolites of Endophytic Bacteria from Glycyrrhiza inflata Bat. of Xinjiang

摘要:

为筛选到可产生甘草酸的内生菌, 从健康的胀果甘草(Glycyrrhiza inflata Bat.)不同组织中分离得到149株内生细菌, 采用发酵培养的方法, 以甘草酸单铵盐为标准品采用TLC法和HPLC法对这些细菌的代谢产物进行筛选, 得到一株可能产甘草酸类似物的内生枯草芽胞杆菌Bacillus subtilis。

Abstract:

Total 149 strains of endophytic bacteria were isolated from different healthy organisms of Glycyrrhiza inflata Bat. from Xinjiang. The fermented liquids of these strains were screened by TLC and analysed by HPLC, and the glycyrrhizinic acid monoammonium salt was taken as standard control at the same time. Only one endophtytic bacterial stain of Bacillus subtilis could produce Glycyrrhizinic acid monoammonium salt analogue.