一株纤维蛋白溶解酶产生菌的鉴定及其产酶条件初步研究

Identification of a Bacillus sp. Strain with Fibrinolytic Activity and Primary Study on Fermentation Process

摘要:

从亚洲传统发酵食品——虾酱中筛选到一株产纤维蛋白溶解酶能力较强的菌株, 通过形态和常规生理生化性质鉴定, 发现该菌株与芽孢杆菌属细菌的特征很相近, 结合16S rDNA 序列分析, 构建系统发育树, 确定其分类地位, 由中国典型培养物保藏中心定名为Bacillus sp. nov. SK006 (CCTCC No. M 205071), 并优化了发酵培养基组成及培养条件, 本研究为该菌株的深入研究和广泛应用提供了理论依据。

关键词:纤维蛋白溶解酶, 芽孢杆菌, 鉴定, 发酵优化

Abstract:

An extracellar fibrinolytic strain was isolated from fermented shrimp paste. In addition to general physiological and biochemical properties, the strain was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain had high similarity with AY601723 and AB195282, suggesting that the strain is a subspecies of Bacillus sp. It was named as Bacillus sp. nov. SK006 by CCTCC. The medium composition and fermentation conditions for fibrinolytic enzyme production were also optimized in the research.

Key words:Fibrinolytic enzyme, Bacillus sp., Identification, Fermentation optimization