青霉TS67菌株活性产物的抗真菌作用

The Antifungal Mechanism of Bioactive Metabolites Produced by Penicillum sp. TS67

摘要:

本文初步探讨了青霉TS67(Penicillum sp.)的发酵活性产物对植物病原真菌的抑制作用机理, 实验结果表明, 用其50%的发酵液分别处理玉蜀黍平脐蠕孢菌(Bipolaris maydis)和大豆尖孢镰刀菌(Fusarium oxysporum)120 h后, 菌丝生长的抑制率分别为77.78%和70.30%, 对孢子产生的抑制率分别达58.8%和73.5%; 同时发现用50%发酵液处理病原菌的无性繁殖孢子12 h后, 孢子萌发抑制率分别达78.3%和62.0%。经显微镜观察抗菌活性物质处理后的菌丝体, 发现菌丝体表面瘤状畸形、菌丝生长顶端不规则膨胀、内部发生原生质浓缩, 初步推测青霉TS67主要通过影响植物病原真菌的细胞壁而实现抑制作用。

Abstract:

The antifungal mechanism of the TS67(Penicillum sp.) producing bioactive metabolites was investigated in this paper. The test indicated that mycelia growth, spores forming and spores germination of Bipolaris maydis and Fusarium oxysporum can be inhibitied by metabolites, after 50% fermentation broth acting 120 hours, the inhibition rate of hyphal growth can reach about 77.78%, 70.30%, the inhibition rate of spores forming also arrived at 58.8%, 73.5%; under 50% fermentation broth acting 12 hours, the inhibition rate of spores germination were up to 78.3%, 62.0%, the mycelia under activity metabolites conditoins also showed surface nodulation and growing point expanding irregularly, protoplasmic condesing, etc. Based on all these results, we may draw a conclusion that activity metabolites maybe target at fungal cell wall.