解磷细菌PSB3的筛选及拮抗作用的研究

The Screening of Phosphorus Solubilizing Bacteria PSB3 and the Study of Its Antagonism

摘要:

利用有机磷细菌液体培养基进行生物富集, 无机磷细菌固体培养基通过平板稀释法进行分离筛选, 建立了土壤解磷细菌的筛选体系。扩增菌株PSB3的16S rDNA序列, 序列测定结果显示, 该片段长度为1525 bp, 经Blastn搜索进行序列比对, 该细菌为洋葱伯克霍尔德氏菌(Burkholderia cepacia)。对该菌株与供试的12个炭疽菌和镰刀菌菌株进行室内拮抗试验, 结果显示, 该菌株对Fusarium solani等6个菌株有不同程度的拮抗作用。

关键词:解磷细菌, 生物富集, 筛选体系, 16S rDNA, 分子鉴定, 拮抗作用

Abstract:

The screening system of phosphorus solubilizing bacteria in the soil was established by biological enriching using organic phosphobacteria liquid medium and then screening via plate dilution method using inorganic phosphobacteria solid medium. By 16S rDNA sequencing technique, the strain PSB3 with the 16S rDNA gene of 1525 bp length was identified as Burkholderia cepacia by Blastn search in NCBI database. The results of in-door antagonism experiment of PSB3 to 12 strains of Fusarium and Colletotrichum (Fusarium solani et al) showed that PSB3 had antagonism reaction against 6 of the 12 pathogens.

Key words:Phosphorus solubilizing bacteria, Biological enrichment, Screening system, 16S rDNA, Molecular identification, Antagonism