荧光原位杂交在喀斯特山地土壤硫酸盐还原菌检测中的应用

Application of Florescence in situ Hybridization to Detecting Karst Mountainous Soil Sulfate-reducing Bacterium

摘要:

采用荧光原位杂交(Florescence in situ Hybridization, FISH) 技术分析了喀斯特山地土壤硫酸盐还原菌(SRB)的数量和空间分布状况。运用16S rRNA特异性探针, 结合DAPI全细胞染色和样品稀释, FISH 技术准确、高效地原位检测出土壤剖面中SRB的数量和分布状况。结果显示, 在所研究的土壤剖面各层均有SRB检出, 最低为(0.8±0.4)×107个/g , 最高为(6.2±1.3)×107个/g, 平均为(2.7±1.2)×107个/g。FISH 能同时对土壤中的SRB进行定性和定量分析, 是研究环境中SRB时空分布的快速有效的检测技术。

Abstract:

Florescence in situ Hybridization(FISH) was used to analyze the amount and spatial distribution of Karst mountainous soil sulfate-reducing bacterium(SRB), 16S rRNA specific probe used with DAPI total cell staining and dilute technology, FISH can in situ detect the amount and spatial distribution of SRB in soil profile efficiently. The results showed that, there were sulfate-reducing bacteria in each layer of soil profile. The minimum cell numbers was (0.8±0.4)×107cells/g and maximum cell numbers was (6.2±1.3)×107cells/g , the average was(2.7±1.2)×107cells/g. FISH is a rapid and effective detection technology in the research of spatial and temporal distribution of SRB in environment that can qualitative and quantitative analysis SRB in the soil simultaneously.