水浮莲中一株西瓜枯萎病拮抗菌的分离、筛选及鉴定

Isolation, Screening and Identification of an Antagonistic Bacterial Against Fusarium oxysporum f. sp. niveum from Pistia stratiotes L.

摘要:

从水浮莲(Pistia stratiotes L.)的叶中分离出1株对西瓜枯萎病有明显拮抗作用的内生细菌XJPL-YB-26, 其发酵液上清在280 nm处有最大紫外吸收峰。利用软件Primer 6.0 设计16S rDNA引物并对其基因组DNA进行扩增并测序得到XJPL-YB-26的部分16S rDNA序列, GenBank接收号为EU251191。经Blastn调出与菌株16S rDNA同源的序列, 并用软件MEGA 3.1按Neighbor- Joining方法构建16S rDNA系统发育树。菌株XJPL-YB-26与AB271744处于同一分支, 相似性为99%, 最终鉴定为枯草芽孢杆菌Bacillus subtilis。

Abstract:

One endophytic bacteria named XJPL-YB-26 had obvious antagonistic action against Fusarium oxysporum f. sp. niveum was isolated from leaves of Pistia stratiotes L., the absorption peak of its metabolizable production is 280 nm. The part 16S rDNA was PCR using the primer designed by Primer 6.0 and sequenced. The accession of GenBank is EU251191. The 16S rDNA phylogenetic tree was constructed by comparing with published 16S rDNA sequences of the relative bacteria species. XJPL-YB-26 and AB271744 was the closest relative with 99% sequence similarity. According to the phylogenetic analysis, it was identified as Bacillus subtilis.