一株新的耐辐射菌WGR702的分离鉴定及耐辐射特性

A New Ionizing-radiation Resistant Strain WGR702 Isolated, Identified, and Radioresistant Character

摘要:

从辐射污染的土壤中分离到一株新的耐辐射菌WGR702。该菌为革兰氏阳性球菌, 直径1.5 μm~2.5 μm。菌体呈粉红色、能运动、兼性厌氧及不产孢子。其生长温度和pH范围分别为10℃~35℃和pH 5.0~10.0。(G+C) mo1%含量为60.5%。UV和γ射线辐射检测表明WGR702具有很强的耐辐射性。16S rDNA序列(EU315117)分析表明, 菌株WGR702与沙雷氏菌属(Serratia)菌株16S rDNA序列有很高相似性(94.79%~98.53%), 但与沙雷氏菌属已报道菌株最大不同点是菌株WGR702细胞为球状, 且革兰氏阳性。结合形态、生理生化特征分析表明菌株WGR702可能是沙雷氏菌属(Serratia)的一个新种。

Abstract:

A new ionizing-radiation resistant strain WGR702 was isolated from arid soils which had been radiated. The strain WGR702 was Gram-positive and coccus, the diameter of the cell was 1.5 μm~2.5μm.The strain WGR702 was pink-pigmented, motile, facultative anaerobe and non-spore forming. The range temperature and pH for strain WGR702 growth were 10℃~35℃ and pH 5.0~10.0 respectively. The strain WGR702 had a G+C content of 60.5 mol%. UV and gamma radiation survival curves showed the strain WGR702 had highly ionizing-radiation resistant. Phylogenetic analysis of the 16S rDNA gene sequences (EU315117) showed 94.79%~98.53% similarities with other recognized Serratia species. Primary characteristics that distinguish isolate WGR702 from the species of genus Serratia include the cells are spherical and Gram-positive. Based on the phenotypic, biochemical and physiological characteristics differences it is proposed that the new isolated strain WGR702 might be classified as a novel species of Serratia.