五氯苯酚厌氧生物降解及降解体系中细菌种群结构分析

Characteristics of PCP Anaerobic Biodegradation and Analysis of Microbial Community Structure in Degrading Consortium

摘要:

研究了不同外加碳源作为共代谢基质及氢气作为电子供体条件下PCP的厌氧生物降解特性, 并借助末端限制性片段长度多态性技术(T-RFLP)分析了PCP降解菌群的微生物群落结构。结果表明, 添加外加碳源及以氢气作为电子供体均对PCP降解有显著促进作用。添加葡萄糖、丙酮酸、酵母膏和氢气时的降解率分别为71%、56%、51%和74%。微生物群落结构分析表明, 不同处理条件下PCP降解菌群微生物群落结构不同。PCP降解菌群中可能存在Clostridium、Frankia和Desulfitobacterium等属的微生物。

Abstract:

Effect of carbon source (glucose, pyruvate, yeast extract) and electron donor (H2) on anaerobic degradation of pentachlorophenol (PCP) was investigated. Differences of bacterial community in the anaerobic systems were also detected by terminal restriction fragment length polymorphism technique (T-RFLP). Compared with carbon-free system, the supplementation of different carbon sources and H2 may greatly enhance the degradation of PCP. The analysis of T-RFLP showed that bacterial community structures were different under different treatment conditions. It was speculated that some microorganisms phylogenetically affiliated with the genus Clostridium, Frankia and Desulfitobacterium existed in the PCP- degradating systems.