PCR-DGGE用于油脂废水处理系统中酵母菌群落结构解析

Analysis of Yeast Community Structure in a Salad Oil-containing Wastewater Treatment System by PCR-DGGE

摘要:

本研究建立了适合于PCR-DGGE分析的环境样品中酵母菌基因组DNA的快速提取方法并利用PCR-DGGE对酵母菌处理油脂废水系统中的酵母菌群落结构进行了解析。结果表明, 并非所有可以降解含油废水中污染物的菌株都可以稳定存在于系统中; 系统对于菌株的选择开始于菌株的扩大培养阶段; 系统从连续运行开始到稳定的过程中, G1、O2或W1成为优势菌株并稳定存在于系统中。

Abstract:

A rapid extraction of yeast genomic DNA suitable for PCR-DGGE analysis was established and the yeast community structure in a salad oil-containing wastewater treatment system was investigated. The results show, not all yeast strains capable of degrading pollutants in wastewater can be planted by system; the selection of system to yeast strains starts from the period of yeast biomass cultivation; from beginning to stable status of continuous wastewater treatment, G1, O2 or W1 are the dominant yeast strains and survive in selection of system.