捕食线虫真菌对松材线虫捕食率测定的新方法

The New Testing Methods of Nematode-trapping Fungi to Pine Wood Nematode Bursaphelenchus xylophilus

摘要:

本文依据捕食线虫真菌的捕食特性以及松材线虫的生长习性, 对松材线虫进行了定时、定量的测定试验, 试图通过培养后线虫的种群数量变化, 来摸索定量测定捕食率的方法。在漏斗法基础上进行了相应的改进, 成功地摸索出3种方法捕食率测定的新方法。各方法各具优缺点, 滤膜镜检法和双层镜检法的数据更精确, 条切镜检法和漏斗法所测数值较粗略。因此, 可依据实验的侧重点以及实验所需的精密程度, 而采用最适宜的方法进行捕食率测定实验。

Abstract:

According to the predacity of the nematode-trapping fungi and the growth habit of pine wood nematode Bursaphelenchus xylophilus, this paper tested the B. xylophilus that cultured by the fixed time and quantities. Trying to find out the quantificational test method of trapping rate, by the change of the quantities of B. xylophilus. Three new methods of testing trapping rate were obtained successfully basing on the improvement of Baermann funnel method. Each method had advantage and disadvantage, Filter membrane and Double layer microscopic examination method were more precise, and bar-cutting microscopic examination and Baermann funnel method were more imprecise. Therefore, according to the emphasis point of experiment and precision degree of the test, the more suitable method for the test of trapping rate could be chosen.