猪源乳酸杆菌对鼠伤寒沙门氏菌DT104在猪肠道上皮细胞IPEC-J2上粘附的影响

Ability of Pig Lactobacilli Strains to Inhibit Salmonella typhimurium DT104 Adhesion and Invasion on Pig Intestinal Epithelial IPEC-J2 Cells

摘要:

采用猪肠道上皮细胞株IPEC-J2体外培养模型, 考察9株猪源乳酸杆菌对IPEC-J2细胞的粘附特性, 以及对鼠伤寒沙门氏菌DT104粘附的竞争、排斥、置换和抗侵袭作用。结果显示, 9株乳酸杆菌均能粘附IPEC-J2细胞, 粘附率在0.1%~10%之间, 具有菌株特异性和浓度效应。乳酸杆菌和沙门氏菌同时加入细胞培养, 能竞争性抑制沙门氏菌的粘附, 并具有浓度效应, 高浓度(109 CFU/mL)添加K30、K67和K16时, 抑制率可达80%以上。乳酸杆菌预处理细胞后再加入沙门氏菌, 高浓度乳酸杆菌可降低沙门氏菌粘附率40%~70%, 而中浓度(108 CFU/mL)乳酸杆菌能抑制23%~33%沙门氏菌对细胞的侵入。但是, 只有高浓度添加乳酸杆菌能置换已经粘附的沙门氏菌, 置换率在12%~84%之间。该结果为临床上筛选乳酸杆菌, 有效防治猪沙门氏菌病提供了一条新的途径。

Abstract:

The adhesion of 9 pig Lactobacillus strains on IPEC-J2 cell line and the effect of 5 Lactobacillus strains on the competitive, exclusive, displacement of adhesion and invasion of Salmonella typhimurium DT104 to IPEC-J2 cells were investigated. The 9 pig Lactobacillus strains exhibited dose- and strain-dependent adherence to intestinal epithelial cells by 0.1%~10%. The ability to inhibit the competitive adhesion of Salmonella typhimurium DT104 appeared to depend on the dose and Lactobacilli tested. Salmonella typhimurium adhesion was reduced above 80% by 109 CFU/mL K30, K67 and K16 strains. Adhesion percent of S. typhimurium DT104 was reduced 40%~70% by pre-treating the cells with 109 CFU/mL Lactobacillus prior to adhesion to IPEC-J2 monolayers, while invasion percent was reduced 23%~33% by 108 CFU/mL Lactobacillus. However, no inhibition of displacement was detected at low concentration of Lactobacillus, while 12%~84% displacement was detected with high concentration. Therefore, the observed inhibition of S. typhimurium adhesion and invasion to IPEC-J2 monolayers with Lactobacillus could be efficient for preventive or curative probiotic therapy in pigs with S. typhimurium disease.