暗纹东方鲀毛霉菌感染的组织病理学观察

Histopathological Observation of Puffer Fugu obscurus Infected with Mucormycosis

摘要:

对病症表现为溃疡性疾病的暗纹东方鲀(Takifagu obscurus)进行了病原菌培养和组织病理观察。镜检溃疡和肌肉组织, 可见丝状型粗大、无隔、分枝, 革兰氏染色阳性的真菌菌丝。心、肝、脾和肠组织有少量炎细胞侵润, 未见真菌菌丝和明显组织病理变化。经Sabouraud培养基培养观察, 37℃, 24 h菌丝顶端有淡黄色球形孢子囊; 72 h出现气生菌丝, 孢子囊成熟, 释放卵圆形孢囊孢子; 120 h后菌落充满平皿。肌体溃疡处皮下和肌肉组织切片镜检显示, 大量菌丝体侵入皮下组织, 炎细胞侵润, 引起邻近肌肉组织变性坏死和间质水肿; 粗大菌丝穿透粘膜入侵基底组织, 同时导致小血管发生栓塞; 菌丝着色显蓝色、分枝、呈直角。依据Ainsworth真菌分类系统鉴定为毛霉属(Mucor sp.)真菌。

Abstract:

Pathogens cultivation and histopathological observation was made on Fugu obscurus with the symptom of ulceration. Microscope examination on ulcers and muscle tissues which infected with fungal mycelium that was coarse, without branch, Gram staining positive. Heart, liver, spleen and intestine contained small amount of inflammatory cell invasion without fungal mycelium and obvious pathological changes. The result of Sabouraud medium culture with the temperature of 37℃ as follow: Mycelia had pale yellow spherical sporangium after 24 h; mycelium appeared, the sporangium matured and oval spores released after 72 h; glass utensil was full of colony after 120 h. Pathological examination of subcutaneous ulcer and muscle revealed that a large number mycelia and inflammatory cell invaded subcutaneous tissue, which caused neighboring muscle tissue degeneration, necrosis and interstitial edema; Coarse hyphae penetrated mucosal into the basement with the result of small vascular embolization at the same time. Mycelium contained blue color and branch looked like a right angle. According to Ainsworth classification system, the pathogen was identified as Mucor sp.