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玉米苗期内生细菌的种群初探及有益内生细菌的筛选
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Preliminary Research on the Flora of Endophytic Bacteria and Selection of Useful Endophytic Bacteria in the Seedling of Maize
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    摘要:

    从3个供试玉米品种(川单13、川单418、川单416)苗期分离、纯化内生细菌68株, 经形态及理化特征鉴定, 分属5个不同的属, 微球菌属和芽孢杆菌属为优势属。芽孢杆菌属细菌鉴定出5个种。供试品种中内生细菌种群数量各不相同, 种群数量由多到少依次是川单13、川单418和川单416。通过抑菌谱和拮抗菌发酵液对种子萌芽率的影响, 筛选出两株抑菌谱广, 对玉米种子萌发无抑制作用的内生芽孢细菌BH和B98。盆栽实验显示, 两株细菌均对玉米植株具有促生作用, 对玉米纹枯病具有防治效果。

    Abstract:

    In the experiment, 68 endophytic bacteria strains of maize were isolated and purified from seedlings of three maize cultivars (Chuandan13, Chuandan418 and Chuandan416). Subsequently, these bacteria were identified and studied. The results indicated that these bacteria belong to five different genera. Among them, Bacillus, Micrococcus was the most widely distributed and predominant. Bacilli were classified into 5 species level. The endophytic bacteria isolated from three maize cultivars were variable, and the number of different species population of these endophytic bacteria followed as chuandan13>chuandan418>chuan- dan416. According to the inhibitory spectrum and influence of endophytic bacteria fermentation to the corn seeds, two strains (BH and B98) had wide pathogen range and didn’t inhibit the viability of the seeds under intraventricular condition; the pot experiment results indicated both of the two strains could promote maize growth and antagonize the Rhizoctonia solani.

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辜运富,张云飞,张小平. 玉米苗期内生细菌的种群初探及有益内生细菌的筛选[J]. 微生物学通报, 2008, 35(7): 1028-1033

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